CLEM/vEM-specific resin R221®

This resin allows for high-contrast electron microscopy images using glutaraldehyde at extremely low concentrations and uranyl acetate, without the need for osmium tetroxide.

It retains fluorescence within the resin.

- Product images (from the top left)

1. Product Image

2-4 Volume electron microscopy images of various samples embedded in R221® resin.

Various samples were high-pressure frozen using the HPM Live μ®, freeze-substituted using 0.05% uranyl acetate, 0.1% glutaraldehyde, and 5% H2O in dry acetone, and then embedded in R221 prior to various image volume imaging methods:
- vEM on the cell monolayer using either a SigmaVP SEM or a Zeiss Crossbeam 540 SEM

2. 3View2XP (Gatan) top view, Sigma VP SEM (Zeiss)

Voltage: 2 kV; High Current: Off; Aperture: 30 μm; Pressure: 8 Pa (N2 gas); Magnification: 7500x; Contrast: 8; Brightness: 5; Frame size: 8192 x 8192; Pixel size: 4.3 nm; Dwell time: 3 μs; Slice thickness: 100 nm

3. Zeiss Crossbeam 540 SEM top view, Atlas 5 for 3D tomography acquisition (Zeiss)

4. Crossbeam Lateral View

Accelerating voltage: 1.5 keV; Current: 1 nA; EsB detector (1200 V grid); Frame size: 4139x1549 (rotated); Pixel size: 5 nm; Slice thickness: 5 nm; Dwell time: 10 μs

CLEM using samples embedded in 5-6 R221® resin

Various samples were high-pressure frozen using the HPM Live μ®, freeze-substituted using 0.05% uranyl acetate, 0.1% glutaraldehyde, and 5% H2O in dry acetone, and then embedded in R221 prior to various image volume imaging methods.
-TEM @ 80kV of a 90nm ultrathin section, without post-staining
- Confocal microscopy, 3View with a Sigma VP, Crossbeam 540, and data merging using eC-CLEM.

Technai 12, 80kV, 90nm thin section. No post-staining.

→ CryoCapCell website

*Please inquire for price and delivery time.